Coding

Part:BBa_K5310009:Design

Designed by: Ilias Pilianidis   Group: iGEM24_thessaloniki   (2024-09-30)


CD28 signalling


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 116
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was retrieved from the NCBI database, the domain's use was evaluated in the literature, and the sequence was codon optimized for expression in human primary cell lines.


Source

The CD28 signalling domain sequence was retrieved from the CD28 gene that resides on chromosome 2 of the human genome.

References